Qiaole Zhao

Biography

I am currently a PhD candidate in Quantitative Imaging Group, Department of Imaging Science and Technology, Faculty of Applied Sciences, Delft University of Technology, Delft, the Netherlands. I received my BS degree in Electronic Engineering from Southeast University, Nanjing, China in 2006. In my master, I majored in microelectronics and solid state electronics, and got my MS degree from Southeast University, Nanjing, China in 2009. My current research interests are on fluorescence microscopy, and FLIM (Fluorescence Lifetime Imaging Microscopy).

Selected Publications

Journal

• Q. Zhao, B. Schelen, R. Schouten, R. van den Oever, R. Leenen, H. van Kuijk, I. Peters, F. Polderdijk, J. Bosiers, M. Raspe, K. Jalink, S. J. G. de Jong, B. van Geest, K. Stoop, I. T. Young, "MEM-FLIM: all-solid-state camera for fluorescence lifetime imagin," Journal of Biomedical Optics, vol. 17(12), pp. 126020-1- 126020-13, 2012.
• Q. Zhao, I. T. Young, and J. G. S. de Jong, "Photon Budget Analysis for Fluorescence Lifetime Imaging Microscopy," Journal of Biomedical Optics, vol. 16(8), pp. 086007-1-086007-16, 2011.
• Q. Zhao, Y. C. Lin, and Q. A. Huang, "A DC Voltage Driven Flow-Through Electroporation Microchip," Advanced Materials Research, 60-61, pp. 44 -48, 2009.
• Q. Zhao, Q. A. Huang, and Y. C. Lin, "Design and Numerical Analysis of a Joule Heating-Induced Continuous-Flow Polymerase Chain Reaction Microchip," Journal of Micro/Nanolithography, MEMS, and MOEMS, 8(2), pp. 021102-1~7, 2009.
• Y. C. Chung, Y. C. Lin, C. D. Chueh, C. Y. Ye, L. W. Lai and Q. Zhao, "Microfluidic Chip of Fast DNA Hybridization Using Denature and Motion of Nucleic Acids," Electrophoresis, 29, pp. 1859-1865, 2008.
• Q. Zhao, "The Design of Simple Transistor Characteristics Display," Electronic Engineer, 32, pp. 13-14, 2006.

Conference

• I. Young, Q. Zhao, B. Schelen, R. Schouten, J. Bosiers, R. Leenen, I. Peters, K. Jalink, M. Raspe, S. de Jong, B. van Geest, "Next-Generation FLIM: Modulated All Solid-State Camera System," XXVIII Congress of the international society for advancement of cytometry, May 19-22, San Diego, United States.
• Q. Zhao, B. Schelen, R. Schouten, R. Leenen, J. Bosiers, M. Raspe, K. Jalink, S. de Jong, B. van Geest, and Ian Ted Young, "Modulated All Solid-State Camera for FLIM," Focus on Microscopy, March 24-27, Maastricht, the Netherlands.
• Q. Zhao, I. T. Young, R. Schouten, S. Stallinga, K. Jalink, S. de Jong, "MB-FLIM: Model-based fluorescence lifetime imaging," Photonics West 2011, January 21-26, 2012, San Francisco, United States.
• Q. Zhao, I. T. Young, B. Schelen, R. Schouten, R. van den Oever, R. Leenen, H. van Kuijk, I. Peters, F. Polderdijk, J. Bosiers, K. Jalink, S. de Jong, B. van Geest, and K. Stoop, "MEM-FLIM: all-solid-state camera for fluorescence lifetime imaging," Photonics West 2011, January 21-26, 2012, San Francisco, United States.
• Q. Zhao, I. T. Young, B. Schelen, R. Schouten, R. van den Oever, H. van Kuijk, I. Peters, F. Polderdijk, J. Bosiers, K. Jalink, S. de Jong, B. van Geest, and K. Stoop, "Modulated All-Solid-State CCD Camera for FLIM," Focus on Microscopy 2011, pp. 278, April 17 - 20, 2011, Konstanz, Germany.
• Q. Zhao, I. T. Young, and J. G. S. de Jong, "Where did my photons go?- Analyzing the measurement precision of FLIM," Proc. of Focus on Microscopy 2010 Conf., pp. 132, Shanghai, China, 2010.
• Q. Zhao, I. T. Young, and J. G. S. de Jong, "Photon budget analysis for a novel fluorescence lifetime imaging microscopy system with a modulated electron-multiplied all-solid-state camera," Proc. of IEEE Nanomed Conf., pp. 25-26, Tainan, Taiwan, 2009.
• Q. Zhao, Y. C. Lin and Q. A. Huang, "Effects of Channel Shapes on Direct-Heated Continuous-Flow Micro Polymerase Chain Reaction," 2nd IEEE International Conference on Nano/Molecular Medicine and Engineering, November 6-9, 2008, Suzhou, China.
• Q. Zhao, Y. C. Lin and Q. A. Huang, "A DC Voltage Driven Flow-Through Electroporation Microchip," 1st International Conference of the Chinese Society of Micro/Nano Technology, November 20-22, 2008, Beijing, China.
• Q. Zhao, Y. C. Lin and Q. A. Huang, "Design and Analysis of Direct-Heated Continuous-Flow Micro-PCR," 2008 Cross Strait Conference on Bio Micro-Nano Technology, June 22-25, 2008, Tainan, Taiwan.
• C. H. Yeh, Q. Zhao and Y.C. Lin, “Using a Novel Method for Generating the UV-Crosslinking Microparticles by Sheath Focusing T-Junction Microfluidic Chip,” The Asia-Pacific Conference of Transducers and Micro-Nano Technology (APCOT 2008), June, 22-25, 2008, Taiwan.
• L. T. Chiou, H. Y. Chen, Q. Zhao, P. Y. Tu and Y. C. Lin, “Using Novel Transdermal Delivery Chip to Transfer Nanoparticles for Skin Permeation,” The Asia-Pacific Conference of Transducers and Micro-Nano Technology (APCOT 2008), June, 22-25, 2008, Taiwan.

Research

My research interest is on fluorescence microscopy, and FLIM (Fluorescence Lifetime Imaging Microscopy).

Fluorescence lifetime imaging (FLIM) is a microscopy tool for in vivo studies of biological cells. By measuring the decay time of fluorescent markers, we can deduce their biochemical and molecular environment. The most accessible technique for FLIM is based on homodyne modulation and demodulation of excitation and emission light in a FLIM system. We are designing, building, and testing a new system based upon a CCD with custom digital electronics that obviates the need for image intensification and fiber optics thus leading to a completely solid-state system. This will improve image quality, SNR, and produce a less-expensive, more-compact system. This will not only increase the applicability of FLIM in cell biology research but should open up new application areas such as medical diagnostics and agriculture.

Contacts

Quantitative Imaging Group
Department of Imaging Science & Technology
Faculty of Applied Sciences
Delft University of Technology
Building 22
Lorentzweg 1- Room F262
2628 CJ Delft
The Netherlands
T: +31-(0)15-278-3221
F: +31-(0)15-278-6740
E: qiaole [dot] zhao [at] tudelft [dot] nl (replace 'at' and 'dot' accordingly)

How to get to my lab?

The most convenient way to reach Delft is to take trains if you are travelling from abroad. Train itineraries can be seen at ns.nl. Once you reach Delft center station, it's around 20 minute walk. The route is shown in the map below. Alternatively, you can take a bus (you can check the route at 9292ov.nl) or a taxi (~10 euro).