Reconstruction from 383 individual super-resolution 2D PAINT images of DNA-origami structures of the TU Delft logo. We obtain wavelength/175 resolution. Each "blob" shows one binding site for fluorophores on the DNA-origmai structure. These sites are 5 nm apart (twice the width of DNA). Image from our publication Heydarian et al. Nature Methods 2018. Click.
Overlay of data fusion from fluorescence super-resolution data of several hundred nuclear pore complexes (NPC) and the negatively stained nuclear envelope of a Xenopus laevis oocyte (sub-sahara frog). Fluorophore Alexa647 is immunostained to the nucleoporin (inner ring 41 nm diameter) and gp210 in the nuclear envelope (outer ring with eight-fold symmetry and 164 nm diameter). The image is generated from data of our publication Löschberger et al. Journal of Cell Sciences 2012. Click.
Infographics of TU Delft logo for super-resolution microscopy. It exemplifies one of the main conclusions from our publication Nieuwenhuizen et al. Nature Methods 2013 where we show that localization uncertainty and density of localizations together determine the interpretable resolution in images beyond the diffraction limit.
Prof.Dr. Bernd Rieger
Department of Imaging Physics
Faculty of Applied Sciences
Delft University of Technology
2628 CJ Delft, The Netherlands
Phone: +31 (0)15 27 88574
Fax: +31 (0)15 27 86740
Building 22, Campus map
Watch our youtube animation about 350 years light microscopy in Delft! It shows the relation of our current research and future direction to the beginns with Antonie van Leeuwenhoek.